Journal: PLoS ONE

Article Title: Field Evaluation of a Point-of-Care CD4 Analyzer for Monitoring HIV Patients in the Interior of the Amazon Region, Brazil

doi: 10.1371/journal.pone.0121400

Figure Lengend Snippet: Comparison between CD4+ T-cell counts produced on paired patient samples by the PIMA POC CD4 analyzer and by conventional FACSCalibur testing, by PIMA POC CD4 testing.

Article Snippet: After shipment to the reference laboratory, venous samples were processed using both the PIMA POC CD4 analyzer and the FACSCalibur (Becton Dickinson Biosciences, San Jose, California, USA).

Techniques: Comparison, Produced

Journal: PLoS ONE

Article Title: Field Evaluation of a Point-of-Care CD4 Analyzer for Monitoring HIV Patients in the Interior of the Amazon Region, Brazil

doi: 10.1371/journal.pone.0121400

Figure Lengend Snippet: Passing-Bablok regression plots: comparison of absolute CD4 counts obtained by the PIMA POC CD4 analyzer using capillary blood samples (A) and venous blood samples (B) versus FACSCalibur using venous blood samples. The solid blue line represents the regression line and the dashed lines represent the 95% CI for the regression line. The corresponding graphs the relative bias between the PIMA POC CD4 analyzer and the FACSCalibur are represented in Pollock plots for capillary blood samples (C) and venous blood samples (D). The solid blue line represent the mean bias. The dashed lines represent the upper and lower limits of agreement (LOA = mean bias ± 1.96 SD) .

Article Snippet: After shipment to the reference laboratory, venous samples were processed using both the PIMA POC CD4 analyzer and the FACSCalibur (Becton Dickinson Biosciences, San Jose, California, USA).

Techniques: Comparison

Journal: PLoS ONE

Article Title: Field Evaluation of a Point-of-Care CD4 Analyzer for Monitoring HIV Patients in the Interior of the Amazon Region, Brazil

doi: 10.1371/journal.pone.0121400

Figure Lengend Snippet: Comparison between CD4+ T-cell counts produced with paired patient samples by the PIMA POC CD4 analyzer and by conventional FACSCalibur testing, by CD4 cell count.

Article Snippet: After shipment to the reference laboratory, venous samples were processed using both the PIMA POC CD4 analyzer and the FACSCalibur (Becton Dickinson Biosciences, San Jose, California, USA).

Techniques: Comparison, Produced

Journal: PLoS ONE

Article Title: Field Evaluation of a Point-of-Care CD4 Analyzer for Monitoring HIV Patients in the Interior of the Amazon Region, Brazil

doi: 10.1371/journal.pone.0121400

Figure Lengend Snippet: Comparison between CD4+ T-cell counts produced with paired patient samples by the PIMA POC analyzer and by conventional FACSCalibur testing, by municipality.

Article Snippet: After shipment to the reference laboratory, venous samples were processed using both the PIMA POC CD4 analyzer and the FACSCalibur (Becton Dickinson Biosciences, San Jose, California, USA).

Techniques: Comparison, Produced

Journal: PLoS ONE

Article Title: Field Evaluation of a Point-of-Care CD4 Analyzer for Monitoring HIV Patients in the Interior of the Amazon Region, Brazil

doi: 10.1371/journal.pone.0121400

Figure Lengend Snippet: Sensitivity, Specificity, NPV, and PPV of capillary PIMA analyzer results versus FACSCalibur at 200 CD4+ T-cell/μl and 500 cell/μl thresholds.

Article Snippet: After shipment to the reference laboratory, venous samples were processed using both the PIMA POC CD4 analyzer and the FACSCalibur (Becton Dickinson Biosciences, San Jose, California, USA).

Techniques:

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: List of CD4 enumeration platforms and antibody reagents tested with commercial stabilized whole blood products.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Stability of Multi-Check , StatusFlow and CD4 Count stabilized whole blood products (SWBPs) under different conditions on various platforms.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Multi-Check , StatusFlow and CD4 Count (low CD4 level) were prepared using the CD3/CD4 reagent kit on product stored at 4°C (D0) and products stored for 1 day at 37°C (D1). Analysis required first setting cursors around the CD3 cells population FSC×CD3 PECy5 dot plot and then isolating the CD4 positive cells cluster on CD4PE×CD3PECy5.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques: